| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roberts, Addyson K (14)
|
VATIDE |
54.55 |
56.15 |
1.60 |
| 2 |
Everhart, Charlotte S (14)
|
VATIDE |
58.67 |
58.90 |
0.23 |
| 3 |
Oluwabusola, Mya Z (13)
|
VATIDE |
58.43 |
59.58 |
1.15 |
| 4 |
Huelsberg, Alexis M (13)
|
VATIDE |
59.75 |
59.90 |
0.15 |
| 5 |
Iniguez, Annalise K (14)
|
VATIDE |
57.86 |
1:00.08 |
2.22 |
| 6 |
Macchi, Lana J (13)
|
VATIDE |
1:00.56 |
1:01.96 |
1.40 |
| 7 |
Fickenscher, Elliot M (13)
|
VATIDE |
59.43 |
1:02.41 |
2.98 |
| 8 |
Goodloe, Lucia S (13)
|
VATIDE |
1:00.91 |
1:03.32 |
2.41 |
| 9 |
Howell, Catherine M (13)
|
VATIDE |
|
1:06.96 |
- |
| 10 |
Hill, Avery M (13)
|
VATIDE |
1:07.17 |
1:08.28 |
1.11 |
| 11 |
Nelson, Rylie G (14)
|
VATIDE |
1:16.38 |
1:11.64 |
-4.74 |